Improved brightfield image segmentation of organoids in Harmony and Image Artist analysis software

Brightfield microscopy serves as the cornerstone for monitoring organoid cultures, offering critical insights into size, morphology, and structural evolution throughout cultivation. In pharmacological research, organoid size variations provide essential readouts for assessing treatment responses and evaluating compound efficacy or toxicity. However, automated brightfield image analysis presents significant technical challenges. Here, we present an enhanced 2.5D image segmentation approach, improving organoid detection and quantification in 3D culture systems.

Scientific poster overview:

• Organoid dome cultures require regular quality control imaging in brightfield channel.
• Brightfield images of dome cultures create significant image analysis challenges.
• Here, we present improvements to previous image segmentation methods that lead to detection of approx. 2x more organoids in dome/hydrogel cultures with significantly improved object splitting.

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For research use only. Not for use in diagnostic procedures.