CRISPR screening enables researchers to investigate the biological activity of tens, hundreds, or even thousands of genes. This method assists in identifying genes that may be essential for various biological pathways, discovering potential drug targets, evaluating probes' functions, stratifying patients, and supporting numerous other important biological applications.
To fully leverage the potential of the screening assay, an initial and crucial step is to select a screening format that aligns with the specific biological questions you are exploring. Here are six common questions our screening services team asks to help guide our customers in choosing the appropriate library format:
- What is the biological question you want to answer?
- Can the phenotype be selected or separated from the background in the cell population?
- Is your facility equipped with lab automation to perform high-throughput screening?
- Is the handling of lentiviral particles allowed?
- How much storage and cell culture space do you have in the facility?
- What do you know about the transfectability and growth characteristics of cells?
Based on these factors, one format may be more appropriate than the other. To clarify, we have outlined various applications, benefits, and considerations for each format.
| Pooled screening: lentiviral | Arrayed screening: synthetic | |
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Expertise throughout your screening processes
Our preclinical team partners with you to design optimal screening strategies.
Arrayed or pooled - no matter which format you choose - we have predefined and custom libraries for CRISPR gene knockout and CRISPR gene modulation applications1-7. The guide RNAs used in our libraries are either validated in the literature, like in our CRISPR mod libraries8 or designed by a functionally validated proprietary algorithm that provides efficient knockout with unparalleled specificity.
Unlock therapeutic targets and biomarkers, from identification to patient stratification with our functional genomic screening services. Alternatively. you can explore our Dharmacon reagents for additional research tools.
References:
- Cluse A, et al. Methods Mol Biol. 1725, 201–227 (2018).
- Pettitt SJ, et al. Nat Commun. 9, 1849 (2018).
- Strezoska Ž, et al. J Biotechnol. 251, 189-200 (2017).
- Tan J, Martin SE. PLoS One 11, e0168968 (2016).
- Costa JR, et al. Assay Guidance Manual (2017).
- le Sage C, et al. Sci Rep. 7(1):17693 (2017).
- Hart T, et al. G3 (Bethesda). 7(8):2719-2727 (2017).
- Horlbeck MA, et al. eLife 5:e19760 (2016).
