HTRF and AlphaLISA SureFire® Ultra no-wash immunoassays as quantitative alternatives to western blot

Move from semi-quantitative to truly quantitative protein analysis.

When your experiments demand precise EC₅₀, IC_50, and DC₅₀, values — not band intensities — western blot's semi-quantitative nature becomes a real bottleneck. HTRF and AlphaLISA™ SureFire® Ultra™ no-wash immunoassays reduce the guesswork, delivering quantitative data from a simplified, plate-based workflow that scales with your science.

• Direct pharmacological parameters -  EC_50, IC_50, and DC₅₀, values extracted from clean dose-response curves across signaling, degradation, and autophagy models.
• Higher sensitivity, lower input - detect basal phosphorylation and low-abundance targets using fewer cells and sub-microgram protein amounts.
• Biologically validated - results align with western blot across 14+ targets and multiple cell models, with most measurements within ±0.3 log units.
• Built for throughput - no wash steps, no gel transfers, no densitometry. Just plate, lyse, detect, and analyze.

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