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Technical Note

AlphaLISA SureFire Ultra: A high performance no-wash assay platform for studying TIE2 and eNOS activation in endothelial cells.

Discover how AlphaLISA™ SureFire® Ultra™ provides robust, quantitative analysis of TIE2–eNOS pathway activation and downstream signaling, even at low cell inputs.

In this technical note, you will learn how to:

  • Detect total and phosphorylated TIE2 (Tyr992) and eNOS (Ser1177) in endogenous endothelial cells using a homogeneous no-wash workflow
  • Characterize dynamic, dose-dependent signaling responses to pathway modulators, including pervanadate and the VE-PTP inhibitor razuprotafib
  • Quantify upstream and downstream pathway components (AKT and ERK) across a wide dynamic range with minimal sample requirements

For research use only. Not for use in diagnostic procedures.

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AlphaLISA SureFire Ultra: A high performance no-wash assay platform for studying TIE2 and eNOS activation in endothelial cells.

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