Cytomegalovirus neutralization assay using different sera measured by direct cell counting in brightfield in a 96-well plate
- The ARPE epithelial host cells are seeded in a 96-well microplate an incubated for 24 hours to allow for the cells to adhere
- The cells are then infected by cytomegalovirus and incubated with titrations of different sera for another 24 hours
- Finally, the cells are fixed and stained with primary anti-viral antibodies and Horseradish Peroxidose (HRP) secondary
- After staining, the cells are imaged and analyzed using the Celigo™ image cytometer to count the number of infected cells
Brightfield-based antibody neutralization assay using HRP
The example below shows the low number of infected cells with high serum concentration in brightfield using HRP
Brightfield image of HRP labeled infected cells with high serum
The example image below shows the high number of infected cells with low serum concentration in brightfield using HRP
Brightfield image of HRP labeled infected cells with low serum
The number of infected cells are counted and dose response curves are generated for 4 different sera, where the IC50 values are calculated. The Celigo is able to output the counted results directly to Microsoft Excel for analysis.
Plate-view of imaged and analyzed wells, and serum dilution-dependent neutralization
plot in respect to the number of HRP-labeled infected cells
For research use only. Not for use in diagnostic procedures.