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Application Support Knowledge Base

Application Support Knowledge Base

The application support knowledge base (ASK) provides a comprehensive set of technology-specific and application-specific scientific articles, written by our R&D, technical support, and applications teams for scientists, enabling you to access scientific support 24 hours a day/7 days a week, with content-rich information covering assay design and assay development, product selection, detailed sample protocols, sample data, data analysis, tips, troubleshooting tables, video tutorials, and selected citations for common assays developed and run using Revvity technologies.

AlphaLISA AlphaScreen
Alpha kinase assays that employ anti-phospho antibodies can be used in both biochemical and cellular assays. These assays can be developed using one of two formats. The direct format assay will use an anti-phospho antibody that is directly conjugated to an Acceptor bead.

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2-Mercaptoethanol and Bead Interference
Adenosine (ATP) and Bead Interference
Alpha Antibody Detection and Characterization
Alpha Bead Conjugation
Alpha cAMP Assays
Alpha Citations
Alpha EMSA Conversion
Alpha Epigenetic Assays
Alpha Epigenetics and Post-translational Modifications
Alpha Immunoassay Design
Alpha Immunoassay Development
Alpha Immunogenicity
Alpha Instrument Options and Settings
Alpha Kinase Anti-Phospho Antibody Assays
Alpha Kinase Assays
Alpha Kinase Substrate-Binding Assays
Alpha Ligand-receptor Assays
Alpha Products and Catalog Numbers
Alpha Protease Assays
Alpha Protein-protein and Protein-nucleic Acid Interactions
Alpha Troubleshooting Tables
AlphaLISA 5 μL vs. 20 μL Sample Volume
AlphaLISA and AlphaScreen No-wash Assays
AlphaLISA Epigenetic Cellular Assays
AlphaLISA Epigenetic Toolbox Reagents
AlphaLISA Immunoassay Kits
Alpha SureFire Assay Development
Alpha SureFire No-wash Cellular Kinase Assays
Anti-6X His AlphaLISA Acceptor Bead Interference
Anti-Chicken IgY AlphaLISA Acceptor Bead Interference
Anti-FITC AlphaLISA Acceptor Bead Interference
Anti-FLAG Donor Bead Interference
Anti-GFP AlphaLISA Acceptor Bead Interference
Anti-Maltose Binding Protein (MBP) AlphaLISA Acceptor Bead Interference
Anti-Mouse IgG Donor Bead Interference
Anti-Mouse IgM AlphaLISA Acceptor Bead Interference
Anti-Rabbit IgG Alpha Donor Bead Interference
Anti-Sheep IgG AlphaLISA Acceptor Bead Interference
Anti-V5 AlphaLISA Acceptor Bead Interference
ASK Alpha Streptactin Interference
Bead Selection and Bead Interference
Buffer Selection for Alpha Assays
Cell-based Protein-protein Interaction Assays
CHAPS and Bead Interference
Citrate and Bead Interference
Create your own Alpha Assay
Data Analysis for AlphaLISA Immunoassays
Deoxycholate and Bead Interference
Design & Development for Alpha Assays That use Tagged Reagents
Design and Development for Alpha Assays That use Tagged Reagents
Design and Development for Alpha Assays That use Tagged Reagents
Determining Kd With an Alpha Assay
DMSO and Bead Interference
DTT and Bead Interference
EDTA and Bead Interference
EGTA and Bead Interference
Gelatin and Bead Interference
Glutathione AlphaLISA
Glycerol and Bead Interference
Glycine and Bead Interference
IBMX and Bead Interference
Imidazole and Bead Interference
Magnesium and Bead Interference
Nickel Chelate AlphaLISA Acceptor Bead Interference
Nitroprusside and Bead Interference
Other Alpha Applications
Preparation of Analyte-depleted Serum for Alpha Assays
Protein A Donor Bead Interference
Protein L AlphaLISA
Roche Protease Inhibitor and Bead Interference
S-adenosylmethionine (SAM) and Bead Interference
SDS and Bead Interference
Sodium Fluoride and Bead Interference
Streptavidin AlphaLISA Acceptor Bead Interference
The Hook Effect
Tris and Bead Interference
Triton X-100 and Bead Interference
Tween 20 and Bead Interference
Urea and Bead Interference
Vanadate and Bead Interference
Working with Cell Extracts and Supernatants in Alpha Assays
Working with Serum and Other Biological Matrices in Alpha Assays

In Vivo Preclinical Imaging
Pre-clinical imaging techniques including  in vivo, ex vivo, and in vitro imaging enable the understanding of disease from cellular to whole animal models and are used in the translation to, and development of therapies in clinical-relevant models.
Cell Counting and Image Cytometry
Nexcelom’s leading automated cell counting and image cytometry solutions are now part of Revvity’s portfolio.The portfolio includes the Celigo™ image cytometer, the Cellaca™ MX high-throughput cell counter, and Cellometer™ automated cell counters. Here you can find information to support your use of these instruments together with our comprehensive range of complementary consumables and reagents.