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Phospho-4E-BP1 (Thr37/46) Terbium Surefire Ultra Detection Kit, 10,000 Assay Points

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-4E-BP1 (Thr37/46) in a multiplexed assay.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: TBSU-P4EBP-A500
Unit Size: 500 Assay Points
List price: USD 1,759.00
Your price:
USD 0.00
USD 1,759.00 /each
Part number: TBSU-P4EBP-A10K
Unit Size: 10,000 Assay Points
List price: USD 10,700.00
Your price:
USD 10,700.00
USD 10,700.00 /each
Part number: TBSU-P4EBP-A50K
Unit Size: 50,000 Assay Points
List price: USD 34,620.00
Your price:
USD 0.00
USD 34,620.00 /each
Part number: TBSU-P4EBP-A-HV
Unit Size: 100 Assay Points
List price: USD 522.40
Your price:
USD 0.00
USD 522.40 /each


Alpha SureFire assays are cellular assays used to detect and quantitate phosphoproteins for cell signaling, pathway analysis, and cellular kinase studies using homogeneous (no wash steps; no separation steps) Alpha technology. Terbium SureFire assays enable multiplexing for detection of two phosphoproteins in a single well, when used in conjunction with any AlphaLISA SureFire Ultra kit. This multiplex measurement is achieved by the use of two types of Alpha Acceptor beads that emit at distinct wavelengths (Terbium: 545 nm, Europium: 615 nm). The two distinct Alpha Acceptor beads report their binding to distinct antigens through their association with specific assay antibodies.

The Terbium “Alpha 545 Acceptor Beads” in the kit are conjugated with a “CaptSure2” agent, which binds a specific CaptSure2 tag on one of the assay antibodies. In combination with the biotinylated antibody provided, the 545 nm signal generated will report levels of the phosphoprotein of interest (Target 2). This kit is to be used in conjunction with the reagents in an AlphaLISA SureFire Ultra kit, which will report that target-of-interest by Europium emission at 615 nm (Target 1).


  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 60 µL reaction volume (30 µL of lysate).
  • Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume (10 µL of lysate).
  • Our 10,000 assay point kit allows you to run 10,000 wells in in 384-well format, using a 20 µL reaction volume (10 µL of lysate).
  • Our 50,000 assay point kit allows you to run 50,000 wells in in 384-well format, using a 20 µL reaction volume (10 µL of lysate).


  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Highly sensitive detection
  • Results in less than 3 hours
  • Half the time of an ELISA assay

*These assays utilize the bead-based Alpha Technology, and requires an Alpha Technology-compatible plate reader capable of reading dual emission wavelengths. Refer to the “AlphaPlex Quick Start Guide” and the “AlphaPlex Assay Development Guide” to find guidance about filters and mirrors selection, instrument protocol and channels crosstalk correction.


Protein Analysis & Detection
Assay Pathway
Assay Points
Assay Target Class
Automation Compatible
AlphaLISA SureFire Ultra Multiplex
Detection Method
Shipping Conditions
Shipped in Blue Ice
Therapeutic Area
Unit Size
10,000 Assay Points

Video gallery


1-4 of 4 Resources
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AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

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AlphaPlex quick start guide

This Quick Start Guide is intended to provide the Alpha user with the knowledge and tools necessary to quickly and easily unleash...

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Alpha SureFire Ultra no-wash immunoassay catalog

Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...

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Measure more analytes in each well.

Now you can quantify multiple analytes in a single well for faster and more accurate results, even with low sample volumes

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SDS, COAs, Manuals and more

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