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DELFIA Tb-N1 Streptavidin, 1mg

Terbium-labeled streptavidin for binding biotinylated biomolecules in DELFIA® time-resolved fluorescence (TRF) assays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: AD0047
Unit Size: 50 µg
List price: USD 436.38
Your price:
USD 0.00
USD 436.38 /each
Part number: AD0048
Unit Size: 1 mg
List price: USD 2,969.00
Your price:
USD 2,969.00
USD 2,969.00 /each

Overview

Generic DELFIA® reagents are intended for use in dissociation-enhanced time-resolved fluorometric assays. All reagents have been labeled using the DELFIA® N1-chelate. They are suitable for highly sensitive end point measurements in separation based assays such as those for detection of protein-protein binding and cell adhesion as well as for immunoassays.

DELFIA® (dissociation-enhanced lanthanide fluorescence immunoassay) is a time-resolved fluorescence (TRF) intensity technology. Assays are designed to detect the presence of a compound or biomolecule using lanthanide chelate labeled reagents, separating unbound reagent using wash steps. DELFIA assays are flexible, compatible with a variety of plate readers, and, as this is a wash-based technology, compatible with most sample types. The technology is based on fluorescence of lanthanide chelates (Europium, Samarium, and Terbium). The fluorescence decay time of these lanthanide chelate labels is much longer than traditional fluorophores, allowing efficient use of temporal resolution for reduction of autofluorescent background. The large Stokes’ shift (difference between excitation and emission wavelengths) and the narrow emission peaks contribute to increasing signal-to-noise ratio. Sensitivity is further increased because of the dissociation-enhancement principle: the lanthanide chelate is dissociated and a new highly fluorescent chelate is formed into a protective micellar solution. DELFIA lanthanide chelates require this dissociation/enhancement step for fluorescence (induced by addition of DELFIA Enhancement solution, DELFIA Inducer, and DELFIA Enhancer as appropriate to the particular lanthanide chelate).

Specifications

Antibody Conjugates
Streptavidin
Application
Protein-protein interactions
Protein Analysis & Detection
Automation Compatible
Yes
Brand
DELFIA
Detection Method
DELFIA TRF
Time-Resolved Fluorescence (TRF)
Experimental Type
In vitro
Fluorophore
Tb-N1
Format
Microplates
One Unit Contains
1.0 mg
Shipping Conditions
Shipped in Blue Ice
Unit Size
1 mg

Resources

1-2 of 2 Resources
Application Note Icon
Application Note
DELFIA Assays: Flexible and Sensitive Tools for Monoclonal Antibody Development

DELFIA® immunoassays are particularly well suited for discovery of specific, high-affinity monoclonal antibodies (mAbs)...

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Guide
Time-resolved fluorometry with the DELFIA method user guide

This guide provides an introduction to the DELFIA method, and to the labeling procedures involved.

SDS, COAs, Manuals and more Illuminator

SDS, COAs, Manuals and more

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