Revvity's AequoScreen double transfected cell lines are provided in two vials of frozen cells, each containing ∽2.5 x 10⁶ cells. Detailed product information as well as recommended cell culture conditions and validation assay date are available each cell line.

AequoScreen technology is a generic GPCR technology that can be used with G_s, G_i, and G_q-coupled GPCRs and calcium coupled ion channels. Following receptor stimulation, increases in intracellular calcium enable measurement of resulting flash luminescence signal.

Features:

• Fully automatable and miniaturizable to 1536
• Enables reduction in reagents and consumable costs
• High signal to background ratio enabling detection of agonists, antagonists and allosteric modulators
• Robust assay (Z′, CV values, solvent resistance)
• Reduces number of false positives from autofluorescent compounds in screening libraries
• Option to work with cells in suspension to increase throughput
• Amenable use with calcium coupled ion channels and tyrosine kinase receptors

Revvity’s AequoScreen cell lines require accepted cell line Terms and Conditions prior to order processing. Additional agreement needed for commercial use. Please use link above to accept Terms and Conditions or to request an agreement for commercial use.