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PhenoVue Fluor 594 - Donkey Anti-Mouse Antibody Cross-Adsorbed
Cross-adsorbed donkey anti-mouse IgG (H+L) antibody conjugated with PhenoVue™ Fluor 594.
PhenoVue Fluor 594 secondary antibody conjugates exhibit bright green fluorescence and are validated for use in high-content screening applications.
Part of Revvity’s portfolio of cellular imaging reagents, the PhenoVue Fluor - 594 donkey anti-mouse antibody, cross-adsorbed, has a maximum excitation wavelength of 590nm and a maximum emission wavelength of 617nm.
View our extensive validation data in the Product Information Sheet within the Resources tab below.
For research use only, not for use in diagnostic procedures.
Product information
Overview
PhenoVue Fluor 594 - donkey anti-mouse IgG (H+L) antibody, cross-adsorbed, is affinity purified and recognizes IgG1, IgG2a, IgG2b and IgG3 isotypes. It can be used in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
PhenoVue Fluor 594 - donkey anti-mouse IgG (H+L) antibody, cross-adsorbed, may display cross-reactivity with other species such as rat or hamster. For improved specificity and lower background, or in multiplexed applications, consider using highly cross-adsorbed PhenoVue Fluor conjugated secondary antibodies as an alternative.
Additional product information
Features
| Numbers of vials per unit | 1 |
|---|---|
| Quantity or volume per unit | 1 mg |
| Form | Lyophilized |
| Storage | 2-8°C |
| Recommended working concentration | 1.5 µg/mL (10nM) |
| Maximum excitation wavelength | 590 nm |
| Maximum emission wavelength | 617 nm |
| Common filter set | Texas-Red |
| Live cell staining | No |
| Fixed cell staining | Yes |
| Equivalent number of microplates | 25-70 x 96-well microplates 20-70 x 384-well microplates 35-90 x 1536-well microplates |
Specifications
| Color |
Red
|
|---|
| Brand |
PhenoVue™
|
|---|---|
| Detection Method |
Fluorescence
|
| Filter |
Texas-Red
|
| Fluorophore |
PhenoVue™ Fluor 594
|
| Host |
Donkey
|
| Product Compatibility |
Fixed samples only
|
| Target Species |
Mouse
|
| Type |
Individual Reagent
|
| Unit Size |
1 vial
|
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Resources
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