Positive control lysate for AlphaLISA™ SureFire® Ultra™ Multiplex Phospho-Ribosomal Protein S6 (Ser240/244)/Total Ribosomal Protein S6 Detection Kit.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
The positive control lysate is prepared from MCF7 cells, cultured to confluence in T175 flasks in 10% FBS containing medium, starved for 2 hours, then treated with 500 µg/mL Insulin for 60 minutes and lysed in 50 mL of 1X SureFire Ultra Lysis buffer.
Application |
Cell Signaling
|
---|---|
Assay Target |
Ribosomal Protein S6
|
Assay Target Class |
Phosphoproteins
|
Assay Technology |
Alpha
|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra Multiplex
|
Detection Method |
Alpha
|
Molecular Modification |
Phosphorylation
|
Product Group |
Control Lysate
|
Shipping Conditions |
Shipped in Blue Ice
|
Unit Size |
1 Vial
|
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