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LANCE Ultra Phospho-STAT5 (Tyr694/699) Detection Kit, 500 Assay Points

LANCE Ultra phospho-STAT5 (Tyr694/699) kits are designed for the detection of STAT5 (phosphorylated at Y694/Y699) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: TRF4009C
Unit Size: 500 Assay Points
List price: USD 2,043.00
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USD 2,043.00
USD 2,043.00 /each
Part number: TRF4009M
Unit Size: 10,000 Assay Points
List price: USD 9,232.00
Your price: Login to view
USD 0.00
USD 9,232.00 /each

Overview

Please note control lysates are NOT included in kits. Control lysates are sold separately, catalog number TRF4009S.

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).

STAT5 is a transcriptional activator which is also involved in intracellular signaling. Activation of STAT5 occurs upon phosphorylation at Tyr694. In some cancers STAT5 is shown to be constitutively activated. Activation can be triggered by a number of ligands including cytokines. STAT5 refers to two proteins with high sequence homology, STAT5A and STAT5B.

Specifications

Application
Kinase
Protein Analysis & Detection
Assay Pathway
JAK/STAT
Assay Target
STAT5
Assay Target Class
Protein
Brand
LANCE Ultra
Detection Method
TR-FRET
Shipping Conditions
Shipped in Blue Ice
Therapeutic Area
Autoimmunity
Respiratory
Unit Size
500 Assay Points

Resources

1-1 of 1 Resources
Application Note Icon
Application Note
LANCE Ultra TR-FRET-based detection and modulation of phosphorylated STAT3 levels in human cells.

STAT3 is a particularly important therapeutic target since it is constitutively active in a range of cancer cells lines and human...

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