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LANCE Ultra Rabbit IgG Detection Kit, 10,000 Assay Points

The LANCE® Ultra Rabbit IgG Detection Kit is designed for detection and quantitation of rabbit IgG in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
  • TRF1104C
    500 Assay Points
  • TRF1104M
    10,000 Assay Points
  • The LANCE® Ultra Rabbit IgG Detection Kit is designed for detection and quantitation of rabbit IgG in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

    • No-wash steps, no separation steps
    • TR-FRET technology
    • Sensitive detection
    • High reproducibility
    • Faster time-to-results
    • Easy automation
    • 96-well, 384-well, and 1536-well formats

    LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

    The glycoprotein immunoglobulin G (IgG), a major effector molecule of the humoral immune response in rabbit, accounts for the majority of the total immunoglobulins in plasma whereas IgM, IgA, and IgE, each of which has characteristic properties and functions, constitute the remainder The basic IgG molecule has a four-chain structure, comprising two identical heavy (H) chains and two identical light (L) chains, linked together by interchain disulfide bonds.. The present kit recognizes total IgGs Fc fragment.

  • Automation Compatible
    Yes
    Application
    Biologics/Bioprocess
    Assay Points
    10000
    Assay Target
    IgG
    Assay Target Class
    Antibody
    Detection Method
    Time-Resolved Fluorescence (TRF)
    TR-FRET
    Experimental Type
    In vitro
    Format
    Microplates
    Brand
    LANCE Ultra
    Quantity in a Package Amount
    1.0 Units
    Unit Size
    10,000 Assay Points