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IVIS Spectrum In Vivo Imaging System

The IVIS® Spectrum in vivo imaging system combines 2D optical and 3D optical tomography in one platform. The system uses leading optical technology for preclinical imaging research and development ideal for non-invasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in living animals.

For research use only. Not for use in diagnostic procedures.

Overview

An optimized set of high efficiency filters and spectral un-mixing algorithms lets you take full advantage of bioluminescent and fluorescent reporters across the blue to near infrared wavelength region. It also offers single-view 3D tomography for both fluorescent and bioluminescent reporters that can be analyzed in an anatomical context using our Digital Mouse Atlas or registered with our multimodality module to other tomographic technologies such as MR, CT or PET.

For advanced fluorescence pre-clinical imaging, the IVIS Spectrum has the capability to use either trans-illumination (from the bottom) or epi-illumination (from the top) to illuminate in vivo fluorescent sources. 3D diffuse fluorescence tomography can be performed to determine source localization and concentration using the combination of structured light and trans illumination fluorescent images. The instrument is equipped with 10 narrow band excitation filters (30nm bandwidth) and 18 narrow band emission filters (20nm bandwidth) that assist in significantly reducing autofluorescence by the spectral scanning of filters and the use of spectral unmixing algorithms. In addition, the spectral unmixing tools allow the researcher to separate signals from multiple fluorescent reporters within the same animal.

Features and Benefits

  • High-sensitivity in vivo imaging of fluorescence and bioluminescence
  • High throughput (5 mice) with 23 cm field of view
  • High resolution (to 20 microns) with 3.9 cm field of view
  • Twenty eight high efficiency filters spanning 430 – 850 nm
  • Supports spectral unmixing applications
  • Ideal for distinguishing multiple bioluminescent and fluorescent reporters
  • Optical switch in the fluorescence illumination path allows reflection-mode or transmission-mode illumination
  • 3D diffuse tomographic reconstruction for both fluorescence and bioluminescence
  • Ability import and automatically co-register CT or MRI images yielding a functional and anatomical context for your scientific data.
  • NIST traceable absolute calibrations
  • Gas anesthesia inlet and outlet ports
  • Class I Laser Product

    Specifications

    Height
    206.0 cm
    Portable
    No
    Width
    65.0 cm
    Brand
    IVIS
    Imaging Modality
    Optical Imaging
    Optical Imaging Classification
    Bioluminescence Imaging
    Fluorescence Imaging
    Quantity in a Package Amount
    1.0 each

    References

    • Guccini et al (2021). Senescence Reprogramming by TIMP1 Deficiency Promotes Prostate Cancer Metastasis. Cancer Cell. 39, 1–15. https://doi.org/10.1016/j.ccell.2020.10.012
    • Ullah et al (2021). Live imaging of SARS-CoV-2 infection in mice reveals neutralizing antibodies require Fc function for optimal efficacy. bioRxiv. https://doi.org/10.1101/2021.03.22.436337
    • Leslie et al (2021). Non-invasive synchronous monitoring of neutrophil migration using whole body near-infrared fluorescence-based imaging. Sci Reports. https://doi.org/10.1038/s41598-021-81097-8
    • Castellarin et al (2020). A rational mouse model to detect on-target, off-tumor CAR T cell toxicity. JCI Insight. https://doi.org/10.1172/jci.insight.136012
    • Wang et al (2020). CRISPR-engineered human brown-like adipocytes prevent diet- induced obesity and ameliorate metabolic syndrome in mice. Sci Transl Med. 12. https://doi.org/10.1126/scitranslmed.aaz8664
    • Zhang et al (2020). A Thermostable mRNA Vaccine against COVID-19. Cell. 182 (5): 1271-1283. https://doi.org/10.1016/j.cell.2020.07.024
    • Hsu et el (2020). Transplantation of viable mitochondria improves right ventricular performance and pulmonary artery remodeling in rats with pulmonary arterial hypertension. J Thor & Cardiovasc Surgery. https://doi.org/10.1016/j.jtcvs.2020.08.014
    • Metman et al (2020). Detection of Papillary Thyroid Cancer Nodal Metastasis after Intravenous Administration of a Fluorescent Tracer Targeting MET. EJSO. 46(2): E12-E13. https://doi.org/10.1016/j.ejso.2019.11.036
    • Gu et al (2020). Rg1 in combination with mannitol protects neurons against glutamate-induced ER stress via the PERK-eIF2 α-ATF4 signaling pathway. Life Sci. 263: 118559. https://doi.org/10.1016/j.lfs.2020.118559
    • Eckley et al (2019). Short-Term Environmental Conditioning Enhances Tumorigenic Potential of Triple-Negative Breast Cancer Cells. Tomography. 5(4): 346–357. https://dx.doi.org/10.18383%2Fj.tom.2019.00019

    Resources

    1-6 of 31 Resources
    Case Study Icon
    Case Study
    A novel mouse model using optical imaging to detect on-target, off-tumor CAR-T cell toxicity

    CAR-T therapy has achieved tremendous success in treating blood malignancies, however treating solid tumors with this therapy has...

    Case Study Icon
    Case Study
    A novel non-Invasive in vivo tool for the assessment of NASH

    Non-alcoholic fatty liver disease (NAFLD) describes a progressive pathology that affects the liver. Fat accumulation causes fatty...

    Technical Note Icon
    Technical Note
    Adaptive Fluorescence Background Subtraction for IVIS systems

    Instrument background occurs when excitation light leaks through the emission filter. This occurs more frequently when the...

    Publication Icon
    Literature - Publication Review
    Applications of In Vivo bioluminescence imaging for SARS-CoV-2

    Scientists continue to explore several options to treat SARS-CoV-2 infection with hundreds of therapeutics at various phases of...

    Technical Note Icon
    Technical Note
    AutoExposure

    This tech note outlines procedures on using auto-exposure on the IVIS® preclinical optical imaging platform using Living Image®...

    Whitepaper Icon
    Whitepaper
    Bioluminescence Resonance Energy Transfer (BRET) to monitor protein-protein interactions

    The ability to image protein-protein interactions (PPIs) in vivo has important implications for a wide variety of biological...

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