HTRF PAb Anti-phospho PKC substrate-Eu cryptate, 500 Assay Points

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HTRF PAb Anti-phospho PKC substrate-Eu cryptate, 500 Assay Points

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Mab Anti-p Pkc Substrate-eu-100,000tests

Product information

For research use only. Not for use in diagnostic procedures.

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Product Variants

partnumber

Part number: 61P03KAE

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Unit Size: 500 Assay Points

List price: USD 872.77

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USD 872.77

USD 872.77 /each

partnumber

Part number: 61P03KAZ

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Unit Size: 10,000 Assay Points

List price: USD 4,888.00

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Product information

Overview

The Anti-phospho PKA-Eu cryptate is a labelled polyclonal antibody. It recognizes phosphorylated serine with arginine or lysine residues in the -2 and +2 positions and a hydrophobic residue in the +1 position. It does not cross-react with non-phosphorylated serine residue or phosphorylated serine in a different configuration, nor does it recognize phospho threonine. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. HTRF allows to perform assay at physiological ATP concentrations.

Specifications

Other Specifications

Assay Points	500
Assay Target Type	Fluorescent reagent
Assay Technology	HTRF
Brand	HTRF
Quantity	1
Shipping Conditions	Shipped in Dry Ice
Therapeutic Area	Cardiovascular Infectious Diseases Inflammation Metabolism/Diabetes NASH/Fibrosis Neuroscience Oncology & Inflammation Rare Diseases
Unit Size	500 Assay Points

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How it works

Assay principle

HTRF kinase assays typically use a combination of a Europium-cryptate labeled anti-phosphoresidue antibody, SA-XL665 and a biotinylated* substrate. The signal is proportional to the concentration of phospho-residues. *Alternatively, other tags such as 6HIS, GST, c-myc, DNP, or FLAG may be used to label the kinase substrate.

HTRF PAb Anti-phospho PKC substrate-Eu cryptate 2.svg

Assay protocol

HTRF kinase assays have two basic phases: 1. Enzymatic step: biotinylated substrate is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated substrate is then detected by the addition of Streptavidin-XL665 and PAb Anti-phospho PKC substrate-Eu cryptate , prepared in a buffer containing EDTA to stop the enzymatic reaction.