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HTRF Mouse CCL2 Detection Kit, 500 Assay Points
HTRF Mouse CCL2 Detection Kit, 500 Assay Points
PHOTO MOUSE CCL2 KIT
The HTRF mouse CCL2 (MCP1) kit is designed for the quantification of mouse CCL2 release in cell supernatant.
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| Feature | Specification |
|---|---|
| Application | Protein Quantification |
| Sample Volume | 16 µL |
The HTRF mouse CCL2 (MCP1) kit is designed for the quantification of mouse CCL2 release in cell supernatant.
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Product Variants
Unit Size: 500 assay points
Part #:
62MCCL2PEG
List Price
USD 1,233.00
Your online price:
Unit Size: 10,000 assay points
Part #:
62MCCL2PEH
List Price
USD 15,202.78
Your online price:
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF Mouse CCL2 Detection Kit, 500 Assay Points
PHOTO MOUSE CCL2 KIT
HTRF Mouse CCL2 Detection Kit, 500 Assay Points
Product information
Overview
Also called MCP-1 (monocyte chemoattractant protein 1), CCL2 is a chemokine mainly secreted by monocytes, macrophages, and dendritic cells to attract monocytes, basophils, and T cells. CCL2 is involved in pathologies like psoriasis or arosclerosis. In brain, CCL2 is released by neurons, astrocytes, and microglia, and is part of neuro-inflammatory response observed in ischemia or Alzheimer's disease. CCL2 is also secreted by adipocytes and can interfere with insulin signaling, thus suggesting a role in inflammation linked to diabetes.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma. When assaying, always follow recommended protocol and avoid highly haemolyzed samples.
Specifications
| Application |
Protein Quantification
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Kit
|
| Sample Volume |
16 µL
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
Cytokines
|
| Target Species |
Mouse
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Metabolism/Diabetes
NASH/Fibrosis
Oncology & Inflammation
|
| Unit Size |
500 assay points
|
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HTRF Mouse CCL2 Detection Kit, 500 Assay Points
HTRF Mouse CCL2 Detection Kit, 500 Assay Points
How it works
Assay principle
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.
Assay data analysis
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting,
Revvity also worked with Myassays.com to help you in your data analysis. you’ll be able to access a free online software to run your CCL2 analysis.
Assay details
Technical specifications of mouse CCL2 (MCP1) kit
| Sample size | 16 µL |
|---|---|
| Final assay volume | 20 µL |
| Kit components | Lyophilized standard, frozen detection antibodies, buffers &protocol |
| LOD &LOQ (in Diluent) | 6 pg/mL & 13 pg/mL |
| Range | 13 – 1,800 pg/mL |
| Time to result | ON at RT |
| Calibration | NA |
| Species | Mouse only |
Analytical performance
Intra and inter assay
Intra assay (n=24)
| Sample | Mean [CCL2] (pg/mL) | CV |
|---|---|---|
| 1 | 173 | 5% |
| 2 | 561 | 2% |
| 3 | 1594 | 2% |
| Mean CV | 3% |
Inter assay (n=4)
| Sample | [CCL2] (pg/mL) | Mean (delta ratio) | CV |
|---|---|---|---|
| 1 | 72 | 722 | 10% |
| 2 | 263 | 3102 | 11% |
| 3 | 960 | 12960 | 16% |
| Mean CV | 12% |
Assay validation
CCL2 secretion in 3T3-L1 cells stimulated with IL1ß and TNFa
3T3-L1 cells* were plated in a 12 well plate at 30,000 cells per well under 1ml. Cells were grown until confluency, and differentiated onto adipocyte (~700,000 cells per well). Differentiated cells were then stimulated for 24h with IL1ß and TNFa, both used at 10ng/ml. Supernatants were diluted 100 times. 16 µL
of diluted supernatants were transferred into a 384 well (SV) white detection plate to be analyzed by the Mouse CCL2 Assay Kit.
Samples were kindly provided by J.F. Tanti’s research team: Cellular and Molecular Physiopathology of Obesity, Inserm U1065, Nice, FRANCE.
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