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HTRF MAb Anti-phospho ELK-1-Eu cryptate, 500 Assay Points

MAb Anti-phospho ELK-1-Eu cryptate enables the rapid detection of ELK phosphorylation on Ser383 in a biochemical kinase assay format.

For research use only. Not for use in diagnostic procedures.

Product Variants
Part number: 61P11KAE
Unit Size: 500 Assay Points
List price: USD 712.27
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USD 712.27
USD 712.27 /each
Part number: 61P11KAZ
Unit Size: 10,000 Assay Points
List price: USD 2,285.00
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USD 0.00
USD 2,285.00 /each

Overview

The Anti-phospho ELK1-Eu cryptate is a labeled monoclonal antibody enabling the rapid detection of ELK1 only when phosphorylated at Ser383, it does not cross-react with the corresponding non-phosphorylated sequence. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. As HTRF tolerates extremely high ATP concentrations, an assay can be performed at physiological ATP concentrations.

Specifications

Assay Points
500
Assay Technology
HTRF
Brand
HTRF
Product Group
Fluorescent Reagent
Quantity
1
Shipping Conditions
Shipped in Dry Ice
Therapeutic Area
Cardiovascular
Infectious Diseases
Inflammation
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Oncology & Inflammation
Rare Diseases
Unit Size
500 Assay Points

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How it works

Assay principle

HTRF kinase assays typically use a combination of a Europium-cryptate labeled anti-phosphoresidue antibody, SA-XL665 and a biotinylated* substrate. The signal is proportional to the concentration of phospho-residues. *Alternatively, other tags such as 6HIS, GST, c-myc, DNP, or FLAG may be used to label the kinase substrate.

 

HTRF MAb Anti-phospho ELK-1-Eu cryptate, 500 Assay Points 1.svg
Assay protocol

HTRF kinase assays have two basic phases: 1. Enzymatic step: biotinylated substrate is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated substrate is then detected by the addition of Streptavidin-XL665 and MAb Anti-phospho ELK-1-Eu cryptate , prepared in a buffer containing EDTA to stop the enzymatic reaction.

HTRF MAb Anti-phospho ELK-1-Eu cryptate, 500 Assay Points 2.svg

Resources

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