AlphaPlex®-545 Acceptor beads conjugated to anti-6XHis antibody. The mouse monoclonal antibody detects proteins containing accessible consecutive histidine residues at the amino or carboxy terminus. These beads can be used to capture His-tagged proteins and peptides, and can be used with Alpha Donor beads to create AlphaPlex no-wash assays for:

• Protein-protein interaction assays
• Protein detection assays
• Enzymatic assays

AlphaPlex Acceptor beads are intended to be used in multiplexing assays, in conjunction with AlphaLISA Acceptor beads and Alpha Donor beads. In a typical Alpha assay:

• 250 μg of Acceptor beads is sufficient to run 250-500 wells using a 50 μL reaction volume
• 5 mg of Acceptor beads is sufficient to run 5,000-10,000 wells using a 50 μL reaction volume
• 25 mg of Acceptor beads is sufficient to run 25,000-50,000 wells using a 50 μL reaction volume