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AlphaPlex 645 (Samarium) Human ICAM-1 Detection Kit, 100 Assay Points

The AlphaPlex™-645 (Sm) Human ICAM-1 Detection Kit is designed for detection and quantitation of human ICAM-1 in cell culture supernatant and human serum using a homogeneous AlphaPlex multiplexing assays (no wash steps).

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Part number: AP282SM-HV
Unit Size: 100 Assay Points
List price: USD 1,014.00
Your price:
USD 1,014.00
USD 1,014.00 /each
Part number: AP282SM-C
Unit Size: 500 Assay Points
List price: USD 4,581.00
Your price:
USD 0.00
USD 4,581.00 /each
Part number: AP282SM-F
Unit Size: 5,000 Assay Points
List price: USD 35,780.00
Your price:
USD 0.00
USD 35,780.00 /each

Overview

The AlphaPlex™-645 (Sm) Human ICAM-1 Detection Kit is designed for detection and quantitation of human ICAM-1 in cell culture supernatant and human serum using a homogeneous AlphaPlex multiplexing assays (no wash steps).

Formats:

  • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

AlphaPlex technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative multiplexing assay. In an AlphaPlex assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaPlex Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 645 nm.

AlphaPlex reagents are intended for use in conjunction with AlphaLISA reagents in multiplexing assays.

Specifications

Application
Multiplexing
Assay Points
100
Assay Target Class
Protein
Automation Compatible
Yes
Brand
AlphaPlex
Detection Method
Alpha
Experimental Type
In vitro
Format
Microplates
Therapeutic Area
Cardiovascular
Unit Size
100 Assay Points

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Guide
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Measure more analytes in each well.

Now you can quantify multiple analytes in a single well for faster and more accurate results, even with low sample volumes

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Application Note
Simultaneous Detection of IL-6 and IL-8 Secretion by Cell Lines using AlphaPlex Technology

This application note describes how AlphaPlex reagents from Revvity can be used to simultaneously detect IL-6 and IL-8 secretion...

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