The AlphaLISA TruHits kit is designed as a tool for AlphaLISA users to identify false positives in AlphaLISA HTS assays early in the screening process, when working with biotin-rich media. This kit includes AlphaLISA Digoxigenin TruHits Acceptor and Anti-Digoxigenin Fab Fragment Donor beads which interact together to generate an AlphaLISA signal. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm. The AlphaLISA Biotin-Free TruHits kit allows the identification of light scattering molecules (insoluble compounds), singlet oxygen quenchers and Digoxigenin mimetics interfering with the AlphaLISA signal, and thus facilitates the detection of false positives. However, TruHits kits do not allow systematic identification of all interfering compounds.