The AlphaLISA Mouse TNFα Biotin-Free Detection Kit is designed for the quantitative detection of mouse TNFα in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Feature | Specification |
---|---|
Application | Protein Quantification |
Dynamic Range | 7 - 70 ng/mL |
Limit of Detection | 0.007 ng/mL |
Limit of Quantification | 0.024 ng/mL |
Sample Volume | 10 µL |
The AlphaLISA Mouse TNFα Biotin-Free Detection Kit is designed for the quantitative detection of mouse TNFα in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Formats:
Features and Benefits:
Tumor Necrosis Factor Alpha is a multifunctional proinflammatory cytokine synthesized mainly by nucleated blood cells as a 233 aa type II transmembrane protein which is cleaved by ADAM17 between aa76-77 to form a soluble homotrimeric complex. TNFα plays a role in lipid metabolism, coagulation and endothelial function and has been associated with cancer, infection and inflammation (including inflammatory bowel disease), ischemia/reperfusion injury and heart failure, and insulin resistance. This kit is used when the analyte is in a matrix containing high levels of biotin, such as can be found in cell culture media such as RPMI, or milk.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA biotin-free assay, a DIG-labeled anti-analyte antibody binds to the anti-DIG-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Application |
Protein Quantification
|
---|---|
Automation Compatible |
Yes
|
Brand |
AlphaLISA
|
Detection Modality |
Alpha
|
Dynamic Range |
7 - 70 ng/mL
|
Limit of Detection |
0.007 ng/mL
|
Limit of Quantification |
0.024 ng/mL
|
Product Group |
Kit
|
Sample Volume |
10 µL
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
TNFα
|
Target Class |
Cytokines
|
Target Species |
Mouse
|
Technology |
Alpha
|
Therapeutic Area |
Inflammation
|
Unit Size |
100 Assay Points
|
Are you looking for resources, click on the resource type to explore further.
AlphaLISA™ technology is a highly sensitive, easy-to-use, and reproducible method for detecting and quantifying molecules in...
We are here to answer your questions.