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AlphaLISA Biotin-Free Human IL-8 Detection Kit, 100 Assay Points

The AlphaLISA™ Human IL8 Biotin-Free Detection Kit is designed for the quantitative detection of human IL-8 in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).

We now offer a new and improved High Performance version of the AlphaLISA Biotin Free Human IL-8 Detection Kit.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Product Variants
Part number: AL328HV
Unit Size: 100 Assay Points
List price: USD 1,094.00
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USD 1,094.00
USD 1,094.00 /each
Part number: AL328C
Unit Size: 500 Assay Points
List price: USD 2,392.00
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USD 0.00
USD 2,392.00 /each
Part number: AL328F
Unit Size: 5,000 Assay Points
List price: USD 15,950.00
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USD 0.00
USD 15,950.00 /each

Overview

Formats:

  • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features and Benefits:

  • No-wash steps, no separation steps
  • Compatible with high biotin samples, including RPMI and high-biotin tissue extracts
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Results in less than 3 hours

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA biotin-free assay, a DIG-labeled anti-analyte antibody binds to the anti-DIG-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Application
Protein Quantification
Assay Target
IL8
Assay Target Class
Cytokines
Assay Technology
Alpha
Automation Compatible
Yes
Brand
AlphaLISA
Detection Method
Alpha
Product Group
Kit
Sample Volume
10 µL
Shipping Conditions
Shipped in Blue Ice
Therapeutic Area
Inflammation
Unit Size
100 Assay Points

Video gallery

Resources

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Application Note
Avoiding biotin interference in AlphaLISA assays

AlphaLISA™ technology is a highly sensitive, easy-to-use, and reproducible method for detecting and quantifying molecules in...

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