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AlphaLISA Human G-CSF Detection Kit, 5,000 assay points

The AlphaLISA® Human Granulocyte Colony-Stimulating Factor (G-CSF) Detection Kit is designed for detection and quantitation of human G-CSF in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: AL213C
Unit Size: 500 Assay Points
List price: USD 2,392.00
Your price:
USD 0.00
USD 2,392.00 /each
Part number: AL213F
Unit Size: 5,000 Assay Points
List price: USD 15,950.00
Your price:
USD 15,950.00
USD 15,950.00 /each

Overview

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Granulocyte colony-stimulating factor, G-CSF (also named Colony Stimulating Factor-3, CSF3), is a 20 kDa cytokine that specifically stimulates the proliferation and differentiation of the progenitor cells of granulocytes. It has been shown to reverse the neutropenia associated with cytotoxic chemotherapy in bone marrow and haemopoietic stem cell transplantation. It’s also a powerful stimulus of resistance to infections, including enhanced phagocytosis, killing of microorganisms, and the production of pharmacologically active products by mature granulocytes and macrophages.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Application
Protein Analysis & Detection
Assay Points
5000
Assay Target Class
Cytokine
Automation Compatible
Yes
Brand
AlphaLISA
Detection Method
Alpha
Experimental Type
In vitro
Format
Microplates
One Unit Contains
5000.0 assay points
Species
Human
Shipping Conditions
Shipped in Blue Ice
Therapeutic Area
Inflammation
Unit Size
5,000 Assay Points

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