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AlphaLISA Human CD28/CD86 Binding Kit, 500 Assay Points

The AlphaLISA® human CD28/CD86 binding kit is designed for the detection of binding activity between human CD28 and CD86, using a fast and simple homogeneous AlphaLISA assay (no wash steps). This assay can be used to screen for small molecules that inhibit binding, as a competitive ligand binding (CLB) assay to screen therapeutic blocking antibodies, and for potency assays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: AL3131C
Unit Size: 500 Assay Points
List price: USD 3,338.00
Your price:
USD 3,338.00
USD 3,338.00 /each
Part number: AL3131F
Unit Size: 5,000 Assay Points
List price: USD 16,580.00
Your price:
USD 0.00
USD 16,580.00 /each

Overview

AlphaLISA features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad range of affinities
  • High avidity
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA® technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

The AlphaLISA human CD28/CD86 binding kit allows determination of cross-reactivity of human anti-CD28 or CD86 reagents with cynomolgus proteins, as well as development or screening of novel human CD28 and CD86 reagents that block the binding of this interaction. Using the AlphaLISA human CD28/CD86 binding assay, small molecule antagonists or large molecule therapeutics and blocking antibodies can be screened in microplate format.

Specifications

Application
Protein Binding
Assay Points
500
Assay Target Class
Protein
Assay Technology
Alpha
Automation Compatible
Yes
Brand
AlphaLISA
Detection Method
Alpha
Experimental Type
In vitro
Species
Human
Shipping Conditions
Shipped in Blue Ice
Therapeutic Area
Immuno-oncology
Unit Size
500 Assay Points

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