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AlphaLISA Human 4-1BB/4-1BBL Detection Kit, 5,000 Assay Points

The AlphaLISA® 4-1BB/4-1BBL binding kit is designed for the detection of binding activity between human 4-1BB and 4-1BBL, using a fast and simple homogeneous AlphaLISA assay (no wash steps). This assay can be used to screen for small molecules that inhibit binding, as a competitive ligand binding (CLB) assay to screen therapeutic blocking antibodies, and for potency assays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: AL3113C
Unit Size: 500 Assay Points
List price: USD 3,365.00
Your price:
USD 0.00
USD 3,365.00 /each
Part number: AL3113F
Unit Size: 5,000 Assay Points
List price: USD 16,790.00
Your price:
USD 16,790.00
USD 16,790.00 /each


The AlphaLISA® human 4-1BB and human 4-1BBL binding assay uses anti-6xHis AlphaLISA Acceptor beads to capture the His-tagged 4-1BB and Streptavidin-coated donor beads to capture the biotinylated 4-1BBL. Donor beads and Acceptor beads come into proximity through 4-1BB binding to 4-1BBL. Excitation of the Donor beads provokes the release of singlet oxygen that triggers a cascade of energy transfer reactions in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.


  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume.
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume.

AlphaLISA features:

  • No-wash steps, no separation steps
  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders

4-1BB is a member of the TNF superfamily. It is a type 2 transmembrane glycoprotein receptor with approximate molecular weight of 30 kDa and expressed mostly on activated T Lymphocytes. The expression is also reported on dendritic cells, B cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation. It has many different names such as CD137 and TNFRSF9. 4-1BB binds with high affinity to the transmembrane 4-1BB Ligand/TNFSF9 which is expressed on antigen presenting cells and myeloid progenitor cells. This interaction co‑stimulates the proliferation, activation, and/or survival of the 4-1BB expressing cells. 4-1BB activation enhances CD8+ T cell and NK cell mediated anti-tumor immunity. Soluble forms of 4-1BB and 4-1BB Ligand circulate at elevated levels in the serum of rheumatoid arthritis and hematologic cancer patients, respectively.


Protein-protein interactions
Assay Points
Assay Target Class
Automation Compatible
Detection Method
Therapeutic Area
Unit Size
5,000 Assay Points

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