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AlphaLISA SureFire Ultra Human Phospho-VEGF Receptor 2 (VEGFR2) (Tyr1175) Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human Phospho-VEGF Receptor 2 (VEGFR2) (Tyr1175) Detection Kit, Control Lysate
Extra control lysate for the AlphaLISA SureFire Ultra phospho-VEGFR2 (Tyr1175) assay. These controls are already provided in the AlphaLISA SureFire Ultra phospho-VEGFR2 assay kit, but can also be ordered separately.
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
Extra control lysate for the AlphaLISA SureFire Ultra phospho-VEGFR2 (Tyr1175) assay. These controls are already provided in the AlphaLISA SureFire Ultra phospho-VEGFR2 assay kit, but can also be ordered separately.
AlphaLISA SureFire Ultra Human Phospho-VEGF Receptor 2 (VEGFR2) (Tyr1175) Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human Phospho-VEGF Receptor 2 (VEGFR2) (Tyr1175) Detection Kit, Control Lysate
Product information
Overview
This control lysate is intended for use with the AlphaLISA SureFire Ultra phospho-VEGF Receptor 2 (Tyr1175) assay kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. This control lysate was prepared from HUVEC cells grown to confluence in T175 flasks, and then serum-starved overnight. The following day the media was removed, and the cells were treated with 100 ng/mL VEGF for 10 minutes. The agonist was removed, and the cells were lysed with 4 mL of freshly prepared 1X Lysis buffer, with shaking for 10 minutes.
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Phosphorylation
|
| Product Group |
Control Lysate
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
VEGFR2
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Unit Size |
1 Vial
|
Video gallery
AlphaLISA SureFire Ultra Human Phospho-VEGF Receptor 2 (VEGFR2) (Tyr1175) Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human Phospho-VEGF Receptor 2 (VEGFR2) (Tyr1175) Detection Kit, Control Lysate
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