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AlphaLISA SureFire Ultra Human TREM2 Aggregation Kit, Control Lysate
AlphaLISA SureFire Ultra Human TREM2 Aggregation Kit, Control Lysate
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human Aggregate TREM2 assay. This control is already provided in the AlphaLISA SureFire Ultra Human Aggregate TREM2 assay kit, but can also be ordered separately.
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human Aggregate TREM2 assay. This control is already provided in the AlphaLISA SureFire Ultra Human Aggregate TREM2 assay kit, but can also be ordered separately.
AlphaLISA SureFire Ultra Human TREM2 Aggregation Kit, Control Lysate
AlphaLISA SureFire Ultra Human TREM2 Aggregation Kit, Control Lysate
Product information
Overview
This control lysate is intended for use with the AlphaLISA SureFire Ultra Human TREM2 Aggregate Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, THP1 cells were seeded at 600K/mL in 10% FBS containing media and treated with 35 ng/mL TGFβ for 20 hours. Cells were then washed with HBSS + 0.1% BSA, adjusted to 4 x 106 cells/mL and treated with 5 μM TREM2 Activator for 30 minutes. Cells were lysed with 5x SureFire Ultra Lysis Buffer.
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Product Group |
Control Lysate
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
TREM2
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Therapeutic Area |
Neuroscience
|
| Unit Size |
1 vial
|
Video gallery
AlphaLISA SureFire Ultra Human TREM2 Aggregation Kit, Control Lysate
AlphaLISA SureFire Ultra Human TREM2 Aggregation Kit, Control Lysate
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