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AlphaLISA SureFire Human Total SYK Detection Kit, Control Lysate
AlphaLISA SureFire Human Total SYK Detection Kit, Control Lysate
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human Total SYK assay. This control is already provided in the AlphaLISA SureFire Ultra Human Total SYK assay kit but can also be ordered separately.
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human Total SYK assay. This control is already provided in the AlphaLISA SureFire Ultra Human Total SYK assay kit but can also be ordered separately.
AlphaLISA SureFire Human Total SYK Detection Kit, Control Lysate
AlphaLISA SureFire Human Total SYK Detection Kit, Control Lysate
Product information
Overview
This control lysate is intended for use with the AlphaLISA SureFire Ultra Human Total SYK Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, U-937 cells were cultured in 10% FBS containing medium. Cells were harvested and washed with HBSS + 0.1% BSA, adjusted to 2 x 106 cells/mL, then treated with 0.5 mM Pervanadate for 30 minutes. Cells were spun down at 300 RCF for 5 minutes and lysed in Lysis Buffer at 1 x 106 cells/mL.
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
SYK
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Unit Size |
1 vial
|
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