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AlphaLISA SureFire Ultra Human and Mouse Phospho-JAK2 (Tyr1008) Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human and Mouse Phospho-JAK2 (Tyr1008) Detection Kit, Control Lysate
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human and Mouse Phospho-JAK2 (Tyr1008) assay. This control is already provided in the AlphaLISA SureFire Ultra Human and Mouse Phospho-JAK2 (Tyr1008) assay kit but can also be ordered separately.
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
| Protocol Time | 2h at RT |
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human and Mouse Phospho-JAK2 (Tyr1008) assay. This control is already provided in the AlphaLISA SureFire Ultra Human and Mouse Phospho-JAK2 (Tyr1008) assay kit but can also be ordered separately.
AlphaLISA SureFire Ultra Human and Mouse Phospho-JAK2 (Tyr1008) Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human and Mouse Phospho-JAK2 (Tyr1008) Detection Kit, Control Lysate
Product information
Overview
This control lysate is intended for use with the AlphaLISA SureFire Ultra Human and Mouse Phospho-JAK2 (Tyr1008) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, HEL92.1.7 cells were seeded at 500K cells/mL and incubated overnight in 10% FBS containing medium. Cells were harvested, washed in HBSS + 0.1% BSA, adjusted to 4 x 106 cells/mL and treated with 0.5 mM Pervanadate for 15 minutes. Following treatment, cells were spun down and lysed with Lysis Buffer to a final concentration of 4 x 106 cells/mL.
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Protocol Time |
2h at RT
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
JAK2
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
Mouse
|
| Technology |
Alpha
|
| Therapeutic Area |
Autoimmunity
Oncology
|
| Unit Size |
1 vial
|
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