Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human Total JAK1 assay. This control is already provided in the AlphaLISA SureFire Ultra Human Total JAK1 assay kit, but can also be ordered separately.
Feature | Specification |
---|---|
Application | Cell Signaling |
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human Total JAK1 assay. This control is already provided in the AlphaLISA SureFire Ultra Human Total JAK1 assay kit, but can also be ordered separately.
This control lysate is intended for use with the AlphaLISA SureFire Ultra Human Total JAK1 Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, HEL92.1.7 cells were seeded at 500K cells/mL and incubated overnight in 10% FBS containing medium. Cells were harvested, washed in HBSS + 0.1% BSA, adjusted to 4 x 106 cells/mL and treated with 0.5 mM Pervanadate for 1 hour. Following treatment, cells were spun down and lysed with Lysis Buffer to a final concentration of 4 x 106 cells/mL.
Application |
Cell Signaling
|
---|---|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Modality |
Alpha
|
Lysis Buffer Compatibility |
Lysis Buffer
|
Molecular Modification |
Total
|
Product Group |
Control Lysate
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
JAK1
|
Target Class |
Phosphoproteins
|
Target Species |
Human
|
Technology |
Alpha
|
Therapeutic Area |
Autoimmunity
Oncology
|
Unit Size |
1 vial
|
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