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AlphaLISA SureFire Ultra Human IRF5 Aggregate Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human IRF5 Aggregate Detection Kit, Control Lysate
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human IRF5 Aggregate assay. This control is already provided in the AlphaLISA SureFire Ultra Human IRF5 Aggregate assay kit but can also be ordered separately.
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
| Protocol Time | 2h at RT |
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human IRF5 Aggregate assay. This control is already provided in the AlphaLISA SureFire Ultra Human IRF5 Aggregate assay kit but can also be ordered separately.
AlphaLISA SureFire Ultra Human IRF5 Aggregate Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human IRF5 Aggregate Detection Kit, Control Lysate
Product information
Overview
This control lysate is intended for use with the AlphaLISA SureFire Ultra Human IRF5 Aggregate Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, THP-1 cells were seeded at 500K cells/mL and incubated overnight in 10% FBS containing medium. Cells were harvested, adjusted to 5 x 106 cells/mL and treated with 100 nM Calyculin A for 3 hours. Following treatment, cells were washed with HBSS + 0.1% BSA and lysed with Lysis Buffer at a final concentration of 10 x 106 cells/mL.
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Protocol Time |
2h at RT
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
IRF5
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Therapeutic Area |
Autoimmunity
Oncology
|
| Unit Size |
1 vial
|
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