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AlphaLISA Surefire Ultra Human Phospho-RIP3 (Ser227) Detection Kit, Control Lysate

Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Phospho-RIP3 (Ser227) (Human) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-RIP3 (Ser227) (Human) assay kit, but can also be ordered separately.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature Specification
Application Cell Signaling

Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Phospho-RIP3 (Ser227) (Human) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-RIP3 (Ser227) (Human) assay kit, but can also be ordered separately.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variant
Unit Size: 1 Vial
Part #:
ALSU-PRIP3-A-L
List Price
USD 142.66

Overview

This control lysate is intended for use with the AlphaLISA SureFire Ultra Human Phospho-RIP3 (Ser227) Assay Kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, U937 cells were seeded at 800 K/mL and grown overnight in T175 flasks in 50 mL of 10% FBS containing medium. Cells were treated with 50 ng/mL TNFα, 20 μM Z-VAD and 200 nM SM-164 for 7 hours at 37°C. Cells were harvested, washed with HBSS and lysed in 5 mL of 1X SureFire Ultra Lysis Buffer.

Specifications

Application
Cell Signaling
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra
Detection Modality
Alpha
Lysis Buffer Compatibility
Lysis Buffer
Molecular Modification
Phosphorylation
Product Group
Control Lysate
Shipping Conditions
Shipped in Blue Ice
Target
RIP3
Target Class
Phosphoproteins
Target Species
Human
Technology
Alpha
Therapeutic Area
Autoimmunity
Central Nervous System
Neuroinflammation
Unit Size
1 Vial

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