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AlphaLISA SureFire Ultra Human Total PI3K p85 Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human Total PI3K p85 Detection Kit, Control Lysate
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human Total PI3K p85 assay. This control is already provided in the AlphaLISA SureFire Ultra Human Total PI3K p85 assay kit but can also be ordered separately.
| Feature | Specification |
|---|---|
| Application | Protein Quantification |
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human Total PI3K p85 assay. This control is already provided in the AlphaLISA SureFire Ultra Human Total PI3K p85 assay kit but can also be ordered separately.
AlphaLISA SureFire Ultra Human Total PI3K p85 Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human Total PI3K p85 Detection Kit, Control Lysate
Product information
Overview
This control lysate is intended for use with the AlphaLISA SureFire Ultra Human Total PI3 Kinase p85 Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, HEK293 cells were cultured to confluence in T175 flasks in 10% FBS containing medium. Cells were starved for 2 hours in serum free media, stimulated with 20 μg/mL Insulin for 5 minutes and then lysed with 4 mL of Lysis Buffer.
Specifications
| Application |
Protein Quantification
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Host Species |
Human
|
| Molecular Modification |
Total
|
| Product Group |
Control Lysate
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
PI3K p85
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Therapeutic Area |
Inflammation
|
| Unit Size |
1 vial
|
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