Extra control lysate for the AlphaLISA™ SureFire® Ultra™ High Performance Human and Mouse Phospho-STAT1 (Tyr701) assay. This control is already provided in the AlphaLISA SureFire Ultra High Performance Human and Mouse Phospho-STAT1 (Tyr701) assay kit but can also be ordered separately.
Feature | Specification |
---|---|
Application | Cell Signaling |
Protocol Time | 2h at RT |
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ High Performance Human and Mouse Phospho-STAT1 (Tyr701) assay. This control is already provided in the AlphaLISA SureFire Ultra High Performance Human and Mouse Phospho-STAT1 (Tyr701) assay kit but can also be ordered separately.
This control lysate is intended for use with the AlphaLISA SureFire Ultra High Performance Human and Mouse Phospho-STAT1 (Tyr701) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, HeLa cells were cultured to confluence in T175 flasks in 10% FBS containing medium. Cells were treated with 0.6 M Sorbitol for 20 minutes followed by 200 ng/mL human IFNγ for a further 20 minutes and then lysed with 6 mL of Lysis Buffer.
Application |
Cell Signaling
|
---|---|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Modality |
Alpha
|
Protocol Time |
2h at RT
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
STAT1
|
Target Class |
Phosphoproteins
|
Target Species |
Human
Mouse
|
Technology |
Alpha
|
Therapeutic Area |
Autoimmunity
Neuroscience
Oncology
|
Unit Size |
1 vial
|
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