Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human Phospho-DAP12 (Tyr91) assay. This control is already provided in the AlphaLISA SureFire Ultra Human Phospho-DAP12 (Tyr91) assay kit, but can also be ordered separately.
This control lysate is intended for use with the AlphaLISA SureFire Ultra Human p-DAP12 (Tyr91) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, THP1 cells were seeded at 400K/mL and grown overnight in T175 flasks in medium containing 10% FBS. Cells were washed in HBSS and treated with 0.25 mM Pervanadate for 1 hour. Cells were lysed in SureFire Ultra Lysis Buffer at a final cell concentration of 5 x 106 cells/mL.
For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Application |
Protein Detection
|
---|---|
Assay Target |
DAP12
|
Assay Technology |
Alpha
|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Method |
Alpha
|
Experimental Type |
In vitro
|
Shipping Conditions |
Shipped in Blue Ice
|
Target Species |
Human
|
Therapeutic Area |
Neuroscience
|
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