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AlphaLISA SureFire Biotin-Free Human and Mouse Phospho-SYK (Tyr525/526) Detection Kit, Control Lysate

Extra control lysate for the AlphaLISA™ SureFire® Biotin-Free Human and Mouse Phospho-SYK (Tyr525/526) assay. This control is already provided in the AlphaLISA SureFire Biotin-Free Human and Mouse Phospho-SYK (Tyr525/526) assay kit but can also be ordered separately.

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Feature	Specification
Application	Cell Signaling
Protocol Time	2h at RT

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Product Variant

Part #:

ASBF-PSYK-A-L

List Price

USD 150.00

Your online price:

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).

AlphaLISA SureFire Ultra Control Lysate image

Product information

Overview

This control lysate is intended for use with the AlphaLISA SureFire Biotin Free Human and Mouse Phospho-SYK (Tyr525/526) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, U937 cells were cultured in 10% FBS containing medium. Cells were harvested, washed with HBSS + 0.1% BSA and adjusted to 2 x 10⁶ cells/mL, then treated with 0.5 mM Pervanadate for 30 minutes. Cells were spun down at 300 RCF for 5 minutes and lysed in Lysis Buffer at 1 x 10⁶ cells/mL.

Specifications

Other Specifications

Application	Cell Signaling
Automation Compatible	Yes
Brand	AlphaLISA SureFire Biotin-Free
Detection Modality	Alpha
Protocol Time	2h at RT
Shipping Conditions	Shipped in Blue Ice
Target	SYK
Target Class	Phosphoproteins
Target Species	Human Mouse
Technology	Alpha
Therapeutic Area	Autoimmunity Oncology
Unit Size	1 vial