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AlphaLISA SureFire Biotin-Free Human Total Rb Detection Kit, Control Lysate
AlphaLISA SureFire Biotin-Free Human Total Rb Detection Kit, Control Lysate
Extra control lysate for the AlphaLISA™ SureFire® Biotin-Free Human Total Rb assay. This control is already provided in the AlphaLISA SureFire Biotin-Free Human Total Rb assay kit but can also be ordered separately.
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
| Protocol Time | 2h at RT |
Extra control lysate for the AlphaLISA™ SureFire® Biotin-Free Human Total Rb assay. This control is already provided in the AlphaLISA SureFire Biotin-Free Human Total Rb assay kit but can also be ordered separately.
AlphaLISA SureFire Biotin-Free Human Total Rb Detection Kit, Control Lysate
AlphaLISA SureFire Biotin-Free Human Total Rb Detection Kit, Control Lysate
Product information
Overview
This control lysate is intended for use with the AlphaLISA SureFire Biotin Free Human Total Rb Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, Jurkat cells were cultured in 10% FBS containing media. Cells were harvested and washed with HBSS + 0.1% BSA, adjusted to 4 x 106 cells/mL and lysed with the addition of 5X Lysis Buffer.
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Biotin-Free
|
| Detection Modality |
Alpha
|
| Protocol Time |
2h at RT
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
Rb
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Therapeutic Area |
Oncology
|
| Unit Size |
1 vial
|
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