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Targeted killing of antigen-positive cancer cells using a high-content live-cell imaging assay

This video, kindly provided by Immunocore, illustrates a high-content live-cell imaging assay which analyses ImmTAC®*- induced killing by T-cells (yellow) of antigen-positive cancer cells (blue).

In the assay, co-cultures of antigen-positive and antigen-negative cancer cells and T-cell effectors (effector to target ratio 1:1) in the presence of ImmTAC® were imaged every 10 min over 375 timepoints (62.5h in total) using the 40X water objective on the Opera™ Phenix™ high-content screening system.

For research use only. Not for use in diagnostic procedures.

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