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Guide

Optimize your GTP binding assays with these Gαi agonist and antagonist guides

Detailed information to help you perform Gαi agonist and antagonist assays effectively!

Written for biologists, researchers, and scientists, these Gαi agonist and antagonist guides provide practical information for optimizing GTP binding, including reagents, workflow, and assays.

The guides feature:

  • A step-by-step approach for performing your HTRF GTP Gi binding assays
  • Comprehensive illustrations and graphs for analyzing critical parameters, such as membrane handling, constitutive activity analysis, key ion effects, and more
  • Insights regarding data analysis
  • Tips for avoiding common pitfalls and speeding up development

For research use only. Not for use in diagnostic procedures.

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Guide
GTP binding assay a guide to optimizing agonists of Gαi
The proper optimization of assay conditions is essential to ensure you obtain the best reagent use and performance. In the case of GPCR signaling, the most suitable assay conditions depend on the GPCR coupling efficacy as well as the pharmacological characteristics of the compound being studied (agonist or antagonist).
Learn more
Guide
GTP binding assay a guide to optimizing antagonists of Gαi
The proper optimization of assay conditions is essential to ensure you obtain the best reagent use and performance. In the case of GPCR signaling, the most suitable assay conditions depend on the GPCR coupling efficacy as well as the pharmacological characteristics of the compound being studied (agonist or antagonist).
Learn more