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Cell Counting and Image Cytometry

Zika Viral Titration with mKate2 Fluorescent Protein Reporter

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Viral Titre
Antibody Binding Inhibition
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Viral Titre
Topic
Zika Viral Titration with mKate2 Fluorescent Protein Reporter
Direct Counting of Individual Infected Cells
High-Throughput Counting Of GFP-Expressing Influenza Viral Foci-1
Influenza Viral Titration With mKate2 Fluorescent Protein Reporter
Zika Viral Titration with mKate2 Fluorescent Protein Reporter
Automated Counting of Plaques

Measure Zika viral titration with mKate2 fluorescent protein reporter in 96-well plate

  1. Vero and Raji host cells are seeded separately in 96-well microplates and allowed to adhere overnight
  2. A titration of 2-folds of Reporter Virus Particles (RVP-Green) are prepared and added to the host cells
  3. The samples are allowed to incubate for 24 hours and then imaged and analyzed with the Celigo™ image cytometer to count the number of infected cells
  4. The Celigo software was first used to identify the Raji cells in the brightfield and Vero cells in the Brightfield and Hoechst fluorescence channel
  5. Next, the GFP fluorescence positive infected cells were directly counted in the green channel
Zika viral titration with mKate2 fluorescent protein reporter-1

Brightfield, fluorescent, and counted images of the total and infected Raji and Vero cells

Zika viral titration with mKate2 fluorescent protein reporter-2

The percent infectivity Celigo results for both Raji and Vero cells are directly compared to FACS flow cytometry showing highly comparable data

For research use only. Not for use in diagnostic procedures.

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