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Cell Counting and Image Cytometry

Influenza Viral Titration With mKate2 Fluorescent Protein Reporter

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Modern Virology Assays
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Sub Section
Viral Titre
Antibody Binding Inhibition
Antibody Neutralization
Cytopathic Effects
Viral Titre
Topic
Influenza Viral Titration With mKate2 Fluorescent Protein Reporter
Direct Counting of Individual Infected Cells
High-Throughput Counting Of GFP-Expressing Influenza Viral Foci-1
Influenza Viral Titration With mKate2 Fluorescent Protein Reporter
Zika Viral Titration with mKate2 Fluorescent Protein Reporter
Automated Counting of Plaques

Measure influenza viral titration with mKate2 fluorescent protein reporter in 96-well plate

  1. MDCK host cells were seeded in a 96-well microplate and allowed to adhere overnight
  2. A titration of 5-folds of mKate2-influenza viral particles were prepared and added to the host cells
  3. The samples were allowed to incubate for 24 hours and then imaged and analyzed with the Celigo™ image cytometer to count the number of infected cells
  4. The Celigo software was used to identify the total MDCK cells in brightfield images, and then using the fluorescent gating function to determine the percent infection rate
Influenza viral titration with mkate2 fluorescent protein reporter-1

Brightfield and fluorescent images of the individual infected cells in the 96-well. The Celigo software gating shows the counting results of mKate2 positive infected cells.

Influenza viral titration with mkate2 fluorescent protein reporter 2

Utilizing the gating functions in the Celigo, the percentage mKate2 positive cells can be measured to generate an infectivity curve

For research use only. Not for use in diagnostic procedures.

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